Guided visual exploration of genomic stratifications in cancer

Marc Streit, Alexander Lex, Samuel Gratzl, Christian Partl, Dieter Schmalstieg, Hanspeter Pfister, Park PJ, and Nils Gehlenborg.

(Nature Methods, 2014.)

Cancer is a heterogeneous disease, and molecular profiling of tumors from large cohorts has enabled characterization of new tumor subtypes. This is a prerequisite for improving personalized treatment and ultimately achieving better patient outcomes. Potential tumor subtypes can be identified with methods such as unsupervised clustering or network-based stratification, which assign patients to sets based on high-dimensional molecular profiles. Detailed characterization of identified sets and their interpretation, however, remain a time-consuming exploratory process. To address these challenges, we combined textquoterightStratomeXtextquoteright, an interactive visualization tool that is freely available at http://www.caleydo.org/, with exploration tools to efficiently compare multiple patient stratifications, to correlate patient sets with clinical information or genomic alterations and to view the differences between molecular profiles across patient sets. Although we focus on cancer genomics here, StratomeX can also be applied in other disease cohorts.

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