Connectomics, a subfield of neuroscience, aims to map and analyze synapse-level wiring diagrams of the nervous system. While recent advances in deep learning have accelerated automated neuron and synapse segmentation, reconstructing accurate connectomes still demands extensive human proofreading to correct segmentation errors. We present SynAnno, an interactive tool designed to streamline and enhance the proofreading of synaptic annotations in large-scale connectomics datasets. SynAnno integrates into existing neuroscience workflows by enabling guided, neuron-centric proofreading. To address the challenges posed by the complex spatial branching of neurons, it introduces a structured workflow with an optimized traversal path and a 3D mini-map for tracking progress. In addition, SynAnno incorporates fine-tuned machine learning models to assist with error detection and correction, reducing the manual burden and increasing proofreading efficiency. We evaluate SynAnno through a user and case study involving seven neuroscience experts. Results show that SynAnno significantly accelerates synapse proofreading while reducing cognitive load and annotation errors through structured guidance and visualization support.

Acknowledgements

This work was supported by NSF award NSF-IIS-2239688 and partially supported by NIH grants 1U01NS132158 and R01HD104969. We gratefully acknowledge Amazon Web Services (AWS) for providing free cloud computing credits, which facilitated the deployment and testing of our system. We also thank the participants of our user and case studies for their valuable contributions.